The SpectraMax® M2 and M2e Multimode Microplate Readers provide two detection modes in one platform and offer the added functionality of bottom-read fluorescence for cell-based assays. Figure 1. Next generation SpectraMax® absorbance microplate readers. This variable pathlength makes it difficult to per-NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. The Gemini XS can read a variety of sample formats from 6- to 384-well microplates in endpoint, kinetic, spectral scan, and well scan modes. This unlocks the transport slide. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. The SPECTRAmax™ PLUS 384 microplate spectrophotometer provides rapid and sensitive UV/VIS measurements of a variety of analytes including specific proteins, nucleic acids, and other molecules across a wide range of concentrations. Chapter 1: Introduction The SpectraMax® Mini Multi-Mode Microplate Reader is a multi-detection instrument with a 6- well to 384-well plate read capability. 303 unable to cal dark current. 1. Plate chamber design of the SpectraMax category 1 microplate readers. If it looks fine, then proceed. Request a Quote!Introduction. However, microplate optimization and read height adjustment should be performed any time a new plate type—or lot of plates—or reagent volume is. Typically the system can detect absorbance, luminescence, fluorescence, and even make more specialized fluorescence measurements like time-resolved fluorescence (TRF) and fluorescence polarization (FP). ¨0112-0115 F 7 Description 1 Introduction The SpectraMax ® M3, M4, M5, and M5 e Microplate Readers are a series of dual-monochromator, multidetection, multi-mode instruments with a triple-mode cuvette port and 6-well to 384-well microplate readingThe SpectraMax iD5 Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. SpectraMax® Plus 384 provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. Align the two slots on the bottle holder with the two pegs on the rail. The calculated LOQ values were 0. With optimized reagents and the industry-leading data acquisition and analysis tool, SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. The Molecular Devices SpectraMax M2e Microplate Reader makes UV-Vis absorbance (200-1000 nm), fluorescence intensity (excitation 250-850 nm, emission 250-850 nm), chemiluminescence intensity (250-850 nm), and. NORTH AMERICA +1 800-635-5577 Mon to Fri 7am - 5pm Pacific Time. It is user upgradeable and budget friendly, and comes with a brochure that provides detailed information on its features, specifications and applications. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of high performance multi-mode capabilities ideal for life science research and drug discovery screening. SPECTRAmax PLUS Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS. SpectraMax M5 reader settings are shown. These settings are also valid for the SpectraMax M3/M4 and FlexStation® 3 Multi-Mode Microplate Readers. Temperature control. HTRF-compatible SpectraMax Multi-Mode Readers. Absorbance (optical density, OD) was read at 570 nm and 605. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Table 1. . By using PathCheck, SpectraMax readers can automatically normalize sample absorbance values to a 1-cm pathlength and calculate the concentrations. MPN:SpectraMax Plus. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate 312 gain calibration failed. For readers without a cuvette port, simply check the box next to ‘PathCheck’. Molecular Devices SpectraMax Plus 384 UV/Vis Microplate Reader. What is the minimum well volume needed to run a 384 well plate? Minimum volume depends on the solution (curved vs. Brochure. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. The system uses two scanning monochromators to determine the optimal excitation and emission settings. Microplate Spectrophotometers. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. The plate reader can measure multiple readouts, including UV. com. The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. With optimized reagents, option to operate the. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. For more sample throughput, the system can SpectraMax iD5; SpectraMax Mini; SpectraMax Mini Install Guide; SpectraMax Mini Reader Plug-In Download Page (required for SoftMax Pro 7. SpectraMax® iD5 Multi-Mode Microplate Reader - Five-Mode Hybrid Microplate Reader with Automatic NFC Filter Identification and Western Blot Capability, Molecular Devices. The data sheet provides detailed information on the system's optical performance, data collection and analysis, plate stacker and robot integration, and validation options. 5 12. The Molecular Devices SpectraMax XPS has an optical design that is the only multi-channel system that truly mimics a dual-beam spectrophotometer. With optimized reagents, option to operate the. Create custom fluorescence assays without having to purchase expensive filter sets. The SpectraMax® M2 and M2e Systems from Molecular Devices are multidetection microplate readers with dualmonochromators, dual-mode cuvette ports, and top- and bottom-reading capability (top-reading only on the M2). SpectraMax M2 and SpectraMax M2e readers can acquire absorbance as well as fluorescence data for samples by issuing a single read command. The Molecular Devices Gemini XPS Fluorescent Microplate Reader provides an ideal environment to determine the optimal excitation and emission settings for most fluorescent assays. These lab workhorses are used in drug discovery, research, bioassay. SpectraDrop High-Throughput Screening (HTS) Kit. The system uses two scanning monochromators to determine the optimal excitation and emission settings. In . Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. Use similar settings for other Molecular Devices readers with fluorescence detection mode. 20 μL of Component A was added to each well of the microplate. The plate was incubated for 10 minutes at room temperature and read at 595 nm on the SpectraMax ABS Plus reader. 100 uL of LAL reagent was then added to the wells containing the endotoxin standards, and the kinetic read was started immediately, using the preconfigured Kinetic Turbidimetric LAL protocol in SoftMax® Pro Software, with. FlexStation® 3 Benchtop Multi-Mode Microplate Reader combines Molecular Devices’ SpectraMax® M5e Multi-Mode Microplate Reader performance with an integrated 8- or 16-channel pipettor into one. 0 out of 5 $ 11,999. SpectraMax iD3 Multi-Mode Microplate Reader Unparalleled performance on a personalized platform The SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The advanced optical and. Fluorescence microplate readers are multimode spectrophotometers with the capability to detect and measure fluorescence emitted by analytes in a multi-well sample format. Users can select from top or bottom read modes for improved sensitivity for solution and cell-based assays. Figure 1. Fill a clean plate with water. Omnia® Compatible Microplate Reader Documentation Version No. Instrument settings can be saved as a protocol file and used repeatedly for reading different microplates or cuvettes. The plate was placed on an shaker for 15 seconds and then incubated for 2 hours at 37°C, 5% CO 2. 500 Euro/Dollars for a basic filter-based ELISA microplate reader and could go up to over 150. These lab workhorses are used in drug discovery. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax ® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. Wide range of applications. Examples of results are presented below for the LMax™ II 384 dedicated, top-of-the-line microplate luminometer, the Analyst ® GT Multi-Mode, Filter-Based Instrument, and the SpectraMax ® M5 Multi-Detection, Monochromator-Based Reader with both top- and bottom-read capability. Introduction. Read one sample or up to 384 in a single plate read using any standard cuvette, or 96- or 384-well microplate. The University of Rouen in Normandy uses the SpectraMax iD3 and. Using the patented PathCheck® Pathlength Measurement Technology, the SpectraMax. microplate reader. See SpectraMax® Microplate Reader User Manual Download Page. Components The main components of SpectraMax and VersaMax readers described in this manual are: • Control panel • Microplate drawerAbsorbance was read at 590 nm on the SpectraMax ABS Plus Microplate Reader. The top- and bottom-reading Gemini EM Microplate Reader with dual monochromators lets users determine the optimal excitation and emission settings for creative new fluorescence. The following document is intended to demonstrate setup of this instrument. Products : General, Microplate Readers. Music: Cylinder Six by Chris Zabriskie is licensed under a Creative Commons Attribution license (microplate reader is a device used to detect chemical, biological, or physical reactions by measuring emitted light. The SpectraMax Quant dsDNA Assay Kits consist of three products, each designed for a specific range of DNA concentrations and optimized for use with fluorescence microplate readers such as the SpectraMax microplate reader (Table 1). Temperature control. The 4 injector configuration enables flash and glow BRET assays and a 2-fold improvement in speed for traditional flash and glow assays. Compact and tunable absorbance microplate readers DATA SHEET. 7°C and the bottom to 36. Page 27 SpectraMax M2 and M2e Microplate Reader User Guide Place a standard 1 cm cuvette that contains the aqueous/solvent mixture you use for the plate samples into the cuvette port. Analytical Technologies Group specializes in the maintenance and repair of Molecular Devices microplate readers, including the SpectraMax® and VersaMax™ absorbance readers. SpectraMax Spectrofluorometers. Positions H21-K22 contained plate blanks of PBS only. The SpectraMax® ABS Plus Microplate Reader can run . The SpectraMax ® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. The SpectraMax® ABS Plus Microplate Reader can run . Chapter5:ReadModesandReadTypes 0112-0115J 27 Microplate Data Acquisition and Analysis Software, users are now able to extend the ease-of-use of their typical plate reader applications to cell-based imaging and western blot detection. A: Microplate Preparation. With optimized reagents and the industry-leading data acquisition and analysis tool SoftMax® Pro 7 Software, the SpectraMax iD3 allows you to customize your workflow to. FI assay. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro Software, and user. The plate reader optics are tunable for absorbance. The Molecular Devices Gemini XS Fluorescent Microplate Reader lets you experiment with a variety of fluorophores and can even find the best excitation and emission wavelengths for new assays. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. Operators can conveniently select up to six wavelengths at a time for absorbance detection in the visible wavelength range of 340 nm – 850 nm. When the incubator is on, the temperature may be controlled from 2°C above ambient to 60°C. following formula: LLD = 3*SD blank *Concentration/(RLU sample-RLU blank). Each sample has a discrete sample beam and reference beam so that each well is measured directly. Type: Clear: Add to Cart. The SpectraMax iD3 reader allows you to interact with your data any way you want, from anywhere you want. microplate reader. The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein quantitation. The Analyst®, FlexStation® and SpectraMax® M5/M5 e from Molecular Devices have received the LanthaScreen® Certified designation from Life Technologies which ensures that these readers are validated to strict standards in instrument setup and assay performance. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path. Our absorbance plate readers feature our PathCheck Sensor technology and. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance and can be equipped to read volumes as low as 2uL in one multimode reader package. 313 reference gain check fail. 2 only) SpectraMax M-series Microplate readers. A new technical approach to platelet aggregation studies. Devices’ StakMax® Microplate Stacker in a matter of minutes and . The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance,. The dual monochromator optics allow. Using the optimized wavelength settings, the SPECTRAmax GEMINI XS fluorescence microplate reader can distinguish between live and dead cells using this assay. 0 out of 5 $ 5,999. SpectraMax L Microplate ReaderThis UV-visible absorbance microplate reader provides ultrafast, full spectral range detection for cuvettes, 96-well, and 384-well microplates, with the only temperature-independent method for pathlength correction. View the LabX ads below to find Molecular Devices SpectraMAX plate readers for sale and. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase reporter gene, G protein-coupled receptor (GPCR) in 96- and 384-well plates. There is the option to add user-installable cartridges as your research needs evolve such as HTRF®,. The plate reader can measure multiple readouts, including UV and visible absorbance. The SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry leading SoftMax Pro. The data suggests that there is little interference from dead cells when live cells are analyzed, however, there may be some interference from live cells when dead cells are analyzed. Absorbance reading with SpectraMax® microplate readers and SoftMax® Pro Software enables optical density measurement of samples in microplate or cuvette format. Here, we demonstrate performance of the SpectraMax® iD3 Multi-Mode Microplate Reader for assays measuring intrinsic tryptophan fluorescence. Italic and boldface type are used for emphasis. Step 2: Launch SoftMax Pro Software. The SpectraMax 340PC384 features correction for varying microplate well volumes and the elimination of standard curves based on PathCheck. Title: SpectraMax i Series Multi-Mode Microplate Readers | Molecular Devices Author: Molecular Devices Subject: SpectraMax i Series offer the ideal solution for any application whether you are looking for an intuitive, easy-to-use reader, multi-user lab, or an upgradeable system that can adapt to support your lab s new projects. Endpoint, kinetic, spectrum. The SpectraMax® M Series Multi-Mode Microplate Readers measure UV and visible absorbance, fluorescence, luminescence, fluorescence polarization, TRF and HTRF. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. SpectraMax i3x Multi-Mode Detection Platform. The SpectraMax M2 and M2e readers can be integrated with the StakMax® Microplate Stacker from Molecular Devices in a matter of minutes. The Gemini EM can sample a variety of formats, from 6- to 384-well. SpectraMax Spectrophotometers for UV-Vis Absorbance Detection. unconventional microplate reader assays, including imaging cytometry and western blots • Protocol sharing with a strong user community • Spectral optimization wizard to maximize assay signal window (SpectraMax i3 and Paradigm readers only) SoftMax Pro Software SoftMax Pro® Software is the most publishedi3x. The SpectraMax® M Series Multi-Mode Microplate Readers are modular and upgradeable with a wide range of high performance capabilities ideal for life science research and. The SpectraMax Injector System with SmartInject™ Technology features low dead volume (10 pL), overflow protection, and ensures. SoftMax® Pro 7. The reader can be equipped with a NanoBRET detection for a 4-fold improvement in limit of. Find our technical support information along with contact details. The SpectraMax® M5 Multi-Mode Microplate Reader delivers single mode reader performance in one multimode reader package. SpectraMax® Gemini™ EM Microplate Reader, Molecular Devices. We would like to show you a description here but the site won’t allow us. It also integrates with the. SPECTRAmax PLUS 384 Microplate Spectrophotometer Operator’s Manual vii Conventions Used in this Manual The names of keys that appear on the SPECTRAmax PLUS 384 control panel are shown in boxed Helvetica type. The SpectraMax i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with user-configurable detection modules for western blot, cell. Microplate readers detect and process biological and chemical data using absorbance (ELISAs, enzyme activity, and nucleic acid and protein quantification), luminescence, and fluorescence detection modes, including intensity, TRF, and polarization. Avoid losing high signal. Microplate readers use a vertical light path so the distance of the light through the sample depends on the volume. 012 fmol 0. 3) This warranty. 6% for the on-board optics (monochromators). Page 28 Chapter 5: Read Modes and Read Types 1. 2 or higher, standard edition only, no GxP) VersaMax™ Microplate Reader (Serial numbers begining with BNxxxxx and BNRxxxxx are supported) SpectraMax® 190 Absorbance Microplate Reader (Serial. western blot membrane detection in a multimode microplate reader platform. Unlike conventional plate readers, multimode microplate readers are able to perform two or more forms of. Products : SpectraMax 250 (Obsolete) SpectraMax® 250 User Manual Download Page. The SpectraMax® i3x Multi-Mode Detection Platform measures spectral-based absorbance, fluorescence, and luminescence with the added functionality of modular upgrades for western blot, imaging, and fast. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Our SpectraTest® ABS2, FL1, LM1, SpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86 The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax L Luminescence Microplate Reader with the variable-volume injectors option (10 µL to 100 µL) can measure both flash and glow luminescence. With three modes of detection for UV/Vis absorbance, fluorescence and luminescence, the SpectraMax® Mini reader is user-upgradeable - from two to three modes, providing researchers with a versatile instrument. Like conventional microplate readers, multimode microplate readers detect reaction signals from microtiter plates in order to perform assays such as ELISA, protein detection, and drug screening assays. ELISA Plate Readers / Microplate Readers. The SpectraMax® Glo Steady-Luc™ Reporter Assay Kit provides a highly sensitive assay for the quan-titation of firefly luciferase expression in mammalian cells. The SpectraMax Plus 384 reader is equipped with the innovative technology patented by Molecular Devices, the PathCheck® Sensor which measures the depth of samples in a microplate through optical path length. To resume collecting kinetic measurements within the same file, press the Append button. Molecular Devices SpectraMax M5 User Manual (86 pages) Multi-Mode Microplate Readers. The SpectraMax Multi-Mode Microplate Readers are packed in a specially designed carton. To maximize sensitivity of the assay, it is necessary to use optimal excitation and emission wavelengths. With the SpectraMax® MiniMax™ 300 Imaging Cytometer, industry-leading SoftMax® Pro. For more detailed information and technical support of Life Technologies assays, please call 1-800-955- 6288 and enter extension 40266 or email drugdiscoverytech@lifetech. Note that this procedure is only for the SpectraMax L model. The SpectraMax® Paradigm Multi- Mode Microplate Reader with Tunable Wavelength (TUNE) Detection Cartridge from Molecular Devices combines the flexibility of wavelength scanning with the high sensitivity of filters. SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Future-proof any laboratory with the SpectraMax® Paradigm® Multi-Mode Microplate Reader. 2 GxP Software is the latest, most secure software to achieve full FDA 21 CFR Part 11 and EudraLex Annex 11 compliance with streamlined workflows to ensure data integrity. The plate is then covered and mixed to allow complete cell lysis before the luminescent signals are read on the SpectraMax i3x Multimode Microplate Reader (Figure 1). The SpectraMax® ABS and ABS Plus absorbance microplate readers provide the flexibility, sensitivity, and convenience for a wide range of assays such as ELISAs, microbial growth, and protein. Chirag Patel, a graduate student at Johns Hopkins, reviewed Molecular Devices' SpectraMax M5 Multi-Mode Microplate Reader and found it an excellent resource for his lab's research on mTOR signaling in CD8 T cells. 58% for the detection module and 2. Narrow 2nm bandwidths deliver increased measurement accuracy and linearity over the widest range. The SpectraMax ® iD5 Multi-Mode Microplate Reader includes an absorbance detection mode optimal for protein quantitation assays. IMAP FP Settings in SoftMax ProSpectraMax M5 Multi-Mode Microplate Reader. Optional enhancements • SpectraMax MiniMax Imaging Cytometer • ScanLater Western Blot System • SpectraDrop™ Micro-Volume Microplate312 gain calibration failed. When read on microplate readers, the SpectraMax Quant dsDNA Assay Kits enable optimal results and increased assay throughput. Preparation of Calibration Standards Calibration Standard (% Phosphorylated) µL Peptide Stock µL Phospho-peptide Stock 0 100 0 12. Every step is optimized for data acquired from a Molecular Devices microplate reader or data imported from. For a higher . Introduction. Microplate Automation & Detection. The fluorometric performance of the SpectraMax M2 e is similar, but slightly superior to that of the Gemini EM, a dedicated top and bottom read fluorescence microplate reader. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. The drawing shows a standard microplate of 15 mm height covered with a lid. , April 14, 2022 – Molecular Devices, LLC. The SpectraMax® is a compact and economical solution designed to save researchers benchtop space and budget. The SpectraMax® M3 Multi-Mode Microplate Reader dual monochromatic optics allows the widest range of applications to be utilized for bioresearch applications all without the need to change filters, and provides three modes of detection in one platform. The plate reader optics are tunable for. For readers without a cuvette port, these options are not shown in the software. 1. This application note describes experimental protocols that enable the detection of relatively low numbers of cells expressing Green Fluorescent Protein. If you do not have an iLab account you must first make one: Spectramax 190 absorbance microplate reader utilizes a patented multichannel optical design to simulate a dual beam spectrophotometer to help eliminate errors through the usage of a dual fiber optic exposure. Molecular Devices Spectramax iD3 Microplate Reader. The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile microplate reader capable of quantitating samples in a variety of microplate formats. Find regional support. Download PDF versions of the SpectraMax Microplate Reader User Guides. Unlike filter-based plate readers, the dual monochromators in the SpectraMax iD5 Multi-Mode Microplate Reader and other SpectraMax readers allow the selection of any wavelength within the reader’s stated range. SpectraMax® Microplate Reader Suite of Products Knowledge Base Published Dec 16, 2020 | Updated Dec 06, 2021 Products : SpectraMax i3x reader,. The SpectraMax® M5e Multi-Mode Microplate Reader delivers single-mode reader performance in one multi-mode reader package. SpectraMax 250 User. Win7 Manual Spectramax-L or StakMax Driver Installation after Initial Install. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 4 μL DNA standards. Water Constant and Cuvette Reference options are available on SpectraMax microplate readers with a cuvette port. Software validation, IQ/OQ/PQ and FDA 21 CFR Part 11 compliance tools are also available. M2. Total volume (except deep. Eight differentSpectraMaxi3xMulti-ModeDetectionPlatformUserGuide 4 5039228D Chapter6:SpectraMax InjectorCartridge 79 InstalltheInjectorCartridge 79 UsingtheInjectorCartridge 86The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. The SpectraMax i3x Multi-mode microplate reader measures spectral-based Absorbance, Fluorescence, and Luminescence with added functaionlity of modular upgrades for Western Blot, Imaging, and Fast Kinetics with Injectors. The SpectraMax® iD5 multi-mode microplate reader is a complete laboratory solution to increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF) and tuneable fluorescence polarisation (FP) read modes. Offer Ends In: 2 Days 10: 40: 19. This technical note demonstrates validation of the SpectraMax® i3x Multi-Mode Microplate Reader for NanoBRET™ assays. Highly customizable low volume. Read More. . 6 for further experiment, so I'm confused which i would choose for OD value determination. Microplate Readers Molecular Devices SpectraMax M2E Microplate Reader. Examples: “Press carefully to engage,” “ Do not. 2) Add in your protocol in Method Editor the Fluorescence Polarization strip. Use the provided 3. The drawing shows a standard microplate of 15 mm height covered with a lid. For biological applications involving small samples, such as purificationThe SpectraMax® iD3 Multi-Mode Microplate Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has launched its SpectraMax® Mini Multi-Mode Microplate. Dual-Glo luciferase assay system (Promega Cat. both cuvette-based and microplate reader applications Microplate Readers. The SpectraMax iD3 reader is a stand-alone multi-mode microplate reader that introduces NFC based user authentication and touchscreen interface personalization. This version is compatible with the following instruments: SpectraMax® Mini Multi-Mode Microplate Reader (software version 7. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable - from 2 to 3 modes, providing researchers with a versatile instrument that. 2 software: see SoftMax® Pro 7. In addition, the SpectraMax® iD5 can be expanded to include bottom. A microplate reader that can detect two or more applications is considered a multi-mode plate reader. 0 CART STOREVersaMax Microplate Reader An affordable, tunable microplate reader for 96-well format Affordable The robot-compatible VersaMax reader is cost-effective—users never have to buy filters. View the Molecular Devices website. 58% for the detection module and 2. The Molecular Devices SpectraMax M5 is a multi-mode plate reader that delivers single mode reader performance with five preferred detection modes for a wide range of applications. The cuvette must be in place when you read the plate. The high sensitivity, low-noise photomultiplier tubes (PMTs), and variable-volume. Contents of wells were pipetted up and down several times to mix. The ABS Plus reader ranges from 190 to 1000 nm in wavelength which is equivalent to having 810 free filters. Download the SpectraMax Mini Reader Plug-in and associated guides. The SpectraMax® ABS Plus Microplate Reader can run . 0 out of 5 $ 2,999-20%. SpectraMax i3/i3x and Paradigm readers can be equipped with the HTRF detection cartridge. SpectraMax and VersaMax Plate Readers OperatorÕs Manual Ñ 0112-0126 Rev A SpectraMax Plus 384 SpectraMax 190 SpectraMax 340PC 384 VersaMax Microplate Spectrophotometer Operator's Manual Molecular Devices Corporation 1311 Orleans Drive Sunnyvale, California 94089 Part # 0112-0126 Rev A. Robust, high-value microplate readers that can run samples based on pre-defined protocols and standard filter modes cover the entire visible range for a variety of assays. Results are shown in Table 1. The SpectraMax® M3, M4, M5 and M5e Multi-Mode Microplate Readers are a modular, upgradeable dual-monochromator microplate reader platform offering a wide range of. Table 1. Page 38 SpectraMax iD3 Multi-Mode Microplate Reader User Guide Bottle Holder The bottle holder is mounted on two knobs for ease of installation, cleaning, and replacement. For more sample throughput, both readersThe SpectraMax M5e Multi-Mode Microplate Reader features a triple-mode cuvette port, temperature control, microplate shaking, and the industry-leading SoftMax Pro. The SPECTRAmax ® GEMINI-XS is a dual-monochromator microplate fluorometer system incorporating highly sensitive optics 4 that permit experimental determinations at low-level detection limits. . Products : SpectraMax i3x reader, SpectraMax iD5 reader, SpectraMax iD3 reader, General, FilterMax F5 reader, SpectraMax ABS reader, SpectraMax L reader, SpectraMax M5/M5e reader This page contains the table of contents of resources available for the SpectraMax Microplate Reader Suite of Products. Compare plate readers The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence. Data exemplifying linear ranges from the three different kits are presented in Figure 2. The instrument uses a paired monochromator and xenon flash. Fluorescence Microplate Reader Comparison Chart. The SpectraMax® absorbance spectrophotometers and plate readers provide the versatility and convenience for a wide range of assays such as ELISAs, nucleic acid and protein quantitation, and microbial growth. Figure 3. The SpectraMax® iD3 and iD5 Multi-Mode Microplate Readers measure absorbance, fluorescence, and luminescence. Set up template in SoftMax Pro and read plate on the SpectraMax M5 microplate reader: Note: IMAP FP protocols for use with FAM- and TAMRA-labeled substrates are available in SoftMax Pro 5 in the Binding Assays protocol folder. With optimized reagents and the industry-leading data acquisition andSpectraMax® Plus 384 microplate reader can run both standard spectrophotometer and microplate reader applications on the same instrument. Similar results can be obtained with the SpectraMax i3x reader and other SpectraMax readers with fluorescence detection. The SpectraMax® i3 is Molecular Devices latest multi-mode microplate reader which delivers several applications including; Ultra-Violet to visible absorbance detection, fluorescence intensity and multi-colour luminescence. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. Unlock your i3/i3x instrument after shipment. Lysozyme contains two tryptophan residues that. The guide also covers the software features, the specifications, and the accessories of the systems. In addition, the SpectraMax iD5Enables data acquisition in endpoint, kinetic, and spectral scan modes at wavelengths across the UV-visible wavelength range, with the only temperature-independent method of pathlength correction. This is equivalent to having 511 built-in filters,The SpectraMax® iD5 Multi-Mode Microplate Reader is the complete laboratory solution to help you increase your research capabilities and comes with built-in absorbance, fluorescence, luminescence, time-resolved fluorescence (TRF), and tunable fluorescence polarization (FP) read modes. begin reading microplates with seven mouse clicks. Microplate automation and detection instruments for walk away operation and high quality results. AlphaScreen assays are compatible with our SpectraMax i3x, SpectraMax i3, and SpectraMax Paradigm Multi-Mode Microplate readers with the Alphascreen Detection Cartridge options. Molecular Devices: Molecular Devices. Table of Contents. The SpectraMax M5 Microplate Reader is the standard for UV/visible multi-mode reader absorbance, providing ultrafast, full spectral range detection for cuvettes, 96-, and 384-well microplates. keeping with best practices, reader performance should. SpectraMax Gemini XPS Microplate Reader A dual-monochromator spectrofluorometer system Dual monochromators With the Gemini XPS Reader, users don’t have to worry about having the right set of filters. The dual monochromator optics allow the widest range of applications to be utilized for bioresearch and drug discovery applications, all without the need to change. 100 fmol 384-well (75 µL) 0. Every SpectraMax® 340 PC 384 microplate reader has an optical system built around a monochromator. 2. The SpectraMax i3x Multi-Mode Microplate Reader has been validated for use with the ValitaTiter and ValitaTiter Plus assays. Figure 3. In this product video, see how the SpectraMax M5 quickly reads samples. Contains one microplate adapter, five 24-well low volume slides, five 64-well low volume bottom slides, and five of each cover slide (2 µL and 4 µL) 0200-6267. View your data quickly using the large touchscreen interface, export your data for analysis in the program of. , a leading provider of high-performance life science solutions and a company of Danaher Corporation (NYSE:DHR), has today launched its. The SpectraMax® ABS Plus Microplate Reader can run both cuvette-based and microplate reader applications on the same instrument. The SpectraMax Mini Multi-Mode Microplate Reader is a compact and versatile instrument that can measure absorbance, fluorescence and luminescence in microplates. The SpectraMax® M3 Multi-Mode Microplate Reader is a UV/visible reader with dual monochromatic optics and three modes of detection for bioresearch procedures. B: Selection of Excitation Wavelength. SpectraMax Plus: SpectraMax Plus. For fluorescence intensity, time resolved fluorescence, and fluorescence polarization assays, the SpectraMax M5 Microplate Reader optical design provides the highest level of flexibility. Download PDF. Find regional support. 380 nm/Exc. When combined with the SpectraDrop Micro-Volume Microplate, the reader can quantitate up to 64 samples with as little as 2 μL sample volume in a single read. . Compact designs. SpectraDrop Micro-Volume Refills, 24-Well Bottom Slides. Supported Plates Microplates having 6, 12, 24, 48, 96, and 384 wells can be used in the SpectraMax Multi-Mode Microplate Readers. SpectraMax L Microplate Reader is a sensitive luminometer with programmable injector options that measures flash and glow luminescence assays, including dual luciferase. The data acquisition and analysis is easily streamlined when using the preconfigured protocol in Softmax Pro Sofware. Category 2 and 3 microplate readers use gradient heating as well. SpectraMax Paradigm Microplate Reader General specifications User-upgradeable Multi-Mode Detection Platform Technical specifications Optimized Guaranteed* Fluorescence Intensity (FI) (Fluorescein) 96-well (200 µL) 0. Page 22 SpectraMax iD3 Multi-Mode Microplate Reader User Guide 2. 000 Euro/Dollars for a high-end multi-mode microplate reader with several detection modes and the best technology for highest sensitivity and fastest detection. Microplate Readers Molecular Devices Spectramax M5 Microplate Reader. It can run samples based on pre-defined protocols and standard filter modes, and is compatible with ELISA and protein quantification assays. Learn about the features, specifications and applications of SpectraMax M2/M2e Microplate Readers, a multi-detection system with dual-mode cuvette ports and dual. The SpectraMax® i3x Multi-Mode microplate reader measures spectral- based Absorbance, Fluorescence, and Luminescence on microplates that range from 6 to 384 wells. The SpectraMax® Mini multi-mode microplate reader offers a budget friendly solution that gives you exactly what you need when you need it. Page 1 SpectraMax M2/M2 user guide ® A MULTI-DETECTION MICROPLATE READER WITH TWO-MODE CUVETTE PORT. Microplate Readers Biotek Epoch Microplate Reader. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. 314 low lamp level warning. microplate reader. Fluorescence Microplate Readers. 5 25 75 25 50 50 50 100 0 100 结合反应 Table 2. Reader is the cornerstone of a complete laboratory solution to help you expand the boundaries of your research capabilities. Microplate reader settings. The SpectraMax® i3x Multi-Mode Microplate Reader measures absorbance, fluorescence, and luminescence with available upgrades including western blot, cell imaging, and fast kinetics with injectors plus additional detection modes. Or scan up to 384 wells at any time to determine the best assay conditions.